comparative study of cellulose tris (3-chloro-5-methylphenylcarbamate) coated or covalently immobilized on silica of for separation of enantiomers in acetonitril in HPLC

Enantiomers represent molecules that are identical structural structures, but have a different spatial orientation of the accumulation of atoms and represent the mirror images of each other. In spite of similar structural formula in the two-dimensional flatness, enantiomers have different biological activity in the burning environment, pharmacokinetics, pharmacodynamics and toxicity. As a rule, in racemic environment only one enantiomer is characterized by pharmacological activity, the second enantiomer prescribes undesirable side effects of the drug and in some cases toxicity. Therefore, Analytical and preparatory partitioning of enantiomers of chiral treatment products is very important. One of the main division method of enantiomer mixtures is the highly effective liquid chromatography, and in a method the most widely used chiral stationary phases are materials prepared based on polysaccharide products. Based on the above, it is important to investigate new stationary phases, which will allow us to divide the broader groups of enantiomers, use the broader groups of solutions, etc. That's why our goal was to use different types of substances like: acid, neutral, and basal nature (which are also subdivisions) Screening and studying on the coated and covalently immobilized cellulose tris columns by using organic solvents,Specifically with acetonitrile on which was added 0,1% DEA for base and neutral substance; and 0,1% FA (formic-acid) for acidic substances.