T cell subsetts and the role of IL-23/Th17 axis in pathogenesis of Psoriasis

Author: oliko nikatchadze
Keywords: Psoriasis, Th17 cells, Cytokines;
Annotation:

Psoriasis is a chronic inflammatory skin disease, which is associated with systemic inflammation and comorbidities, such as psoriatic arthritis and cardiovascular diseases and is among the most frequent T cell mediated disorders affecting about 2% of the population worldwide. Common cause of Psoriasis are environmental factors, infectious disease, antibiotics and genetic predisposition. Recently has established autoimmune nature of Psoriasis and different subclasses of T cells are playing key role in immunopathogenesis of the disease. Including Th1 and Th17 cells which are involved in initiation and amplification of the skin inflammation process, furthermore in cases of recurring Psoriasis Th22 cells are playing the role of memory cells, with the help of Th9 cells, which also are involved in this process. The main goal of our study is to evaluate the role of IL-23/Th17 axis in the patients with Psoriasis in Georgian population. Based on the complex nature of the disease it has been important to identify the cells and cytokines which are leading the process. We have estimated the number of Th17, Th22, Th9 cells in blood samples as in the case of patients suffering from psoriasis and healthy controls. Also, we focused on the activation of IL-23/Th17 axis by evaluating the level of IL-17A, IL-17F, IL-22 and IL-9. We evaluated the role of IL-23/Th17 axis with association of proinflammatory cytokines. We investigated untreated the peripheral blood of newly diagnosed patients with active Psoriasis. There were used the Flow cytometry for immunophenotyping and ELISA methodology. According this research there was not shown dramatic increasing of Th17 cells in the peripheral blood of the patients with Psoriasis. The9 cells were decreased and Th22 cells were increased compared to the control group. The activation, expansion, homing markers and memory cells phenotyping had the different results. There was increased the expression of chemokine receptors (CCR4, CCR6, CCR10) and memory cells surface molecules. As a conclusion, we can say that the T cellular and cytokine profile balance of IL-23/Th17 axis in peripheral blood (unlike the local psoriatic inflammation) is not characterized for Psoriasis.