Temperature dependence of Separation of flurbirofen enantiomers using amylose tris(3,5-dimethylphenylcarbamate) coated on silica gel as stationary phase in High-Performance Liquid Chromatography

Author: Tamari tskhadadze
Keywords: Chiral, Enthalpy, Entropy, Isostantelectric temperature
Annotation:

A chiral molecule and its mirror image are called enantiomers. The enantiomers have same physical and chemical properties in achiral environment and only way to distinguish between them in such environment is the sign of rotation angle of polarized light. The identity of physical and chemical properties makes a separation of enantiomers as very challenging task. The enantiomers of chiral biologically active compounds often have very different physiological properties; this makes enantioseparation of pharmaceuticals, food additives, agrochemicals and other biologically active compounds very important. The goal of our research was the calculation of thermodynamic parameters of enantioseparation in high-performance liquid chromatography. This can help to predict the suitable analytical conditions for the given compound on given column and to achieve the desired selectivity and enantiomer elution order. Firstly we analyzed 28 acidic compounds, from these results we choose four chiral compounds, on particular: Fenoprofen, Flurbiprofen, Ibuprofen and Ketoprofen. In this thesis only the behavior of one chiral substance- Flurbiprofen is described.