Temperature dependence of Separation of Ibuprofen enantiomers using amylose tris(3,5-dimethylphenylcarbamate) coated on silica gel as stationary phase in High-Performance Liquid Chromatography

Author: Gizo Dolidze
Keywords: Enantiomers, Thermodinamic parameters, temperature influence
Annotation:

A molecule whose composition includes the asymmetric atom (asymmetric centre) is the chiral molecule. the chiral molecule is not compatible with it’s non-superposable mirror image. A chiral molecule and its mirror image are called enantiomers. The enantiomers have same physical and chemical properties in achiral environment and only difference in them is the sign of rotation angle of polarized light and because of this its very difficult to analyse them. Despite that enantiomers have same physical and chemical properties, difference in them is only their physiological activity. This makes enantioseparation of pharmaceuticals, food additives, agrochemicals and other biologically active compounds very important. Nowadays high-performance liquid chromatography known as one of the fastest and sensitive method for separation of enantiomers. The goal of our research was the calculation of thermodynamic parameters of enantioseparation in high-performance liquid chromatography. This helps us to predict the suitable analytical conditions for the given compound on given column and to achieve the desired selectivity and enantiomer elution order. Study was conducted on different temperature and mobile phases. For analyzed compounds, the difference in adsorption enthalpy and adsorption entropy were calculated using the plot of natural logarithm of the separation factor versus the reciprocal of the absolute temperature. We studied if the enantioseparation mechanism was controlled by enthalpic or entropic term and what was the influence of temperature on retention and separation.